Communications biology · 2025
Enhanced genome editing with a Streptococcus equinus Cas9.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Liu Jingtong, Wang Yao, Wei Jingjing, Wang Shengzhou, Li Miaomiao, Huang Zheyong, Zhang Sufang, Liu Huihui, Huang Jinhai, Wang Yongming
Аннотация
A large number of SpCas9 orthologs has been computationally identified, but their genome editing potential remains largely unknown. In this study, a GFP-activation assay was used to screen a panel of 18 SpCas9 orthologs, ten of which demonstrated activity in human cells. Notably, these orthologs had a preference for purine-rich PAM sequences. Four of the tested orthologs displayed enhanced specificity compared to SpCas9. Of particular interest is SeqCas9, which recognizes a simple NNG PAM and displays activity and specificity comparable to SpCas9-HF1. In addition, SeqCas9 exhibits superior base editing efficiency compared to SpCas9-NG and SpCas9-NRRH at multiple endogenous loci. This research sheds light on the diversity of SpCas9 orthologs and their potential for specific and efficient genome editing, especially in cases involving base editing.