European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2008
Genetic characterization of the UCS and Kex1 loci of Pneumocystis jirovecii.
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Esteves F, Tavares A, Costa M C, Gaspar J, Antunes F, Matos O
Аннотация
Nucleotide variation in the Pneumocystis jirovecii upstream conserved sequence (UCS) and kexin-like serine protease (Kex1) loci was studied in pulmonary specimens from Portuguese HIV-positive patients. DNA was extracted and used for specific molecular sequence analysis. The number of UCS tandem repeats detected in 13 successfully sequenced isolates ranged from three (9 isolates, 69%) to four (4 isolates, 31%). A novel tandem repeat pattern and two novel polymorphisms were detected in the UCS region. For the Kex1 gene, the wild-type (24 isolates, 86%) was the most frequent sequence detected among the 28 sequenced isolates. Nevertheless, a nonsynonymous (1 isolate, 3%) and three synonymous (3 isolates, 11%) polymorphisms were detected and are described here for the first time.
Предметные рубрики MeSH
Bronchoalveolar Lavage FluidConserved SequenceDNA, FungalFungal ProteinsHIV SeropositivityHumansMolecular Sequence DataPneumocystis cariniiPneumonia, PneumocystisPolymerase Chain ReactionPolymorphism, GeneticPortugalSequence AlignmentSequence Analysis, DNASerine EndopeptidasesSputumTandem Repeat Sequences