Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases · 1999
[Studies on allelic zymogram of periodic Brugia malayi from Zhejiang Province].
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Deng S, Wang P, Zeng X, Yan X, Chen C
Аннотация
AIM: To study the allelic zymogram of periodic B. malayi from Zhejiang Province.
METHODS: 180 adult worms(male 60, female 120), 32,000 microfilaria and 1,500 infective larvae of periodic B. malayi were examined for the isoenzymes of 14 enzymes by horizontal starch gel electrophoresis with 14 different isomyme.
RESULTS: Twenty-seven allelic loci were found in three developing stages of B. malayi, most of them were homozygotes, however, two of them(MPI,MDH) were heterozygotes(7.4%). 17, 19 and 9 loci were presented in adult worms, microfilariae and infective larvae, respectively. At the same time, the allelic zymogram of B. malayi from Guizhou Province was also examined.
CONCLUSION: The allelic zymogram of periodic B. malayi from Zhejiang Province was similar to that of periodic B. malayi from Guizhou Province but passaged in the laboratory.
METHODS: 180 adult worms(male 60, female 120), 32,000 microfilaria and 1,500 infective larvae of periodic B. malayi were examined for the isoenzymes of 14 enzymes by horizontal starch gel electrophoresis with 14 different isomyme.
RESULTS: Twenty-seven allelic loci were found in three developing stages of B. malayi, most of them were homozygotes, however, two of them(MPI,MDH) were heterozygotes(7.4%). 17, 19 and 9 loci were presented in adult worms, microfilariae and infective larvae, respectively. At the same time, the allelic zymogram of B. malayi from Guizhou Province was also examined.
CONCLUSION: The allelic zymogram of periodic B. malayi from Zhejiang Province was similar to that of periodic B. malayi from Guizhou Province but passaged in the laboratory.