Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2020
[The expression and significance of interleukin-35 receptor in oral lichen planus].
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Zhang Ling-Yan, Wang Rong, Liu Ting, Cai Yang
Аннотация
OBJECTIVE: To investigate the expression of glycoprotein 130 (gp130) and interleukin 12 receptor β2 (IL-12Rβ2) in two subunits of interleukin-35 receptor (IL-35R), singal transducer and activator of transcription (STAT) 1 and STAT4 in oral lichen planus (OLP) tissues, and to explore the role and significance of IL-35R in the formation and development of OLP lesions.
METHODS: Totally 41 samples of OLP tissues (OLP group) and 15 samples of normal oral mucosa (control group) were collected. The expression levels of gp130, IL-12Rβ2, STAT1, STAT4 mRNA in the tissues were detected by real-time fluorescent quantitative polymerase chain reaction and the distribution and expression of protein gp130 and IL-12Rβ2 were detected by immunohistochemistry. The potential relationship between gp130 and IL-12Rβ2 and clinical features of OLP was analyzed.
RESULTS: 1) The expression levels of gp130, IL-12Rβ2, STAT1 and STAT4 mRNA in the OLP group were significantly higher than those in the control group (P<0.05). 2) The positive expression rates of gp130 and IL-12Rβ2 protein in the OLP group were higher than those in the control group (P<0.05). The expression of gp130 and IL-12Rβ2 proteins in OLP tissues were positively correlated (r=0.984, P<0.001). 3) The expression rates of gp130 and IL-12Rβ2 protein in erosive OLP tissues were significantly higher than those in non-erosive ones (P<0.05).
CONCLUSIONS: The expression of IL-35R and STAT is up-regulated in OLP tissues, and the expression of IL-35R is related to the clinical classification of OLP, suggesting that IL-35R might play an important role in the formation and development of damage OLP lesions.
METHODS: Totally 41 samples of OLP tissues (OLP group) and 15 samples of normal oral mucosa (control group) were collected. The expression levels of gp130, IL-12Rβ2, STAT1, STAT4 mRNA in the tissues were detected by real-time fluorescent quantitative polymerase chain reaction and the distribution and expression of protein gp130 and IL-12Rβ2 were detected by immunohistochemistry. The potential relationship between gp130 and IL-12Rβ2 and clinical features of OLP was analyzed.
RESULTS: 1) The expression levels of gp130, IL-12Rβ2, STAT1 and STAT4 mRNA in the OLP group were significantly higher than those in the control group (P<0.05). 2) The positive expression rates of gp130 and IL-12Rβ2 protein in the OLP group were higher than those in the control group (P<0.05). The expression of gp130 and IL-12Rβ2 proteins in OLP tissues were positively correlated (r=0.984, P<0.001). 3) The expression rates of gp130 and IL-12Rβ2 protein in erosive OLP tissues were significantly higher than those in non-erosive ones (P<0.05).
CONCLUSIONS: The expression of IL-35R and STAT is up-regulated in OLP tissues, and the expression of IL-35R is related to the clinical classification of OLP, suggesting that IL-35R might play an important role in the formation and development of damage OLP lesions.