An investigation of the squamous cell epithelium of the human larynx by the method of tissue culture.

Laryngeal squamous cell epithelium has been grown by the method of tissue culture. Explant outgrowths were examined initially. Subsequently colonies of epithelium were grown from single cells upon a feeder layer of irradiated 3T3 mouse fibroblasts in a suitable growth medium. Human prepuce, which is widely used in skin culture studies, served as a control. There were colonial differences between colonies of the squamous epithelium of the larynx which presented a loose lattice structure and those of the prepuce control which gave rise to compacted growth. However, no obvious differences were noted between squamous epithelial colonies derived from fetal and adult vocal cords epithelium.