[Study of graft-versus -leukemia activity of gamma-ray irradiated haplomismatched donor lymphocytes infusion].

OBJECTIVE

To explore graft-versus-leukemia activity of 7.5 Gy gamma-ray irradiated haplomismatched donor lymphocytes infusion (DLI).

METHODS

L615 cells loaded 615 (H2(k))/BALB/c (H-2(d)) F(1) mice were infused with irradiated haplo-mismatched donor lymphocytes at 3, 5, 7 days after CTX (200 mg/kg) treatment which was given at the 3rd day after L615 cells inoculation.

RESULTS

T-cells irradiated with 7.5 Gy gamma-ray lost their proliferative capacity but preserved their cytotoxic activity against allogeneic targets. Survival of these recipient mice was longer than that of control (t = 10.03, P < 0.001) with no severe graft-versus-host disease (GVHD).

CONCLUSION

7.5 Gy gamma irradiation attenuated the GVHD inducing potential of allogeneic lymphocytes while preserving their graft-versus-leukemia activity. It provides a simple modality to postpone and prevent leukemia relapse.