Pharmacogenomics · 2015
Development of a simple genotyping method for the HLA-A*31:01-tagging SNP in Japanese.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Maekawa Keiko, Nakamura Ryosuke, Kaniwa Nahoko, Mizusawa Seiho, Kitamoto Aya, Kitamoto Takuya, Ukaji Maho, Matsuzawa Yumiko, Sugiyama Emiko, Uchida Yoshimi, Kurose Kouichi, Ueta Mayumi, Sotozono Chie, Ikeda Hiroko, Yagami Akiko, Matsukura Setsuko, Kinoshita Shigeru, Muramatsu Masaaki, Ikezawa Zenro, Sekine Akihiro, Furuya Hirokazu, Takahashi Yukitoshi, Matsunaga Kayoko, Aihara Michiko, Saito Yoshiro, Japan Pharmacogenomics Data Science Consortium
Аннотация
AIM: To construct a simple, low-cost typing method for the surrogate marker of HLA-A*31:01, a risk factor for carbamazepine (CBZ) related Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN).
MATERIALS & METHODS: DNAs from Japanese SJS/TEN patients were used for genotyping and developing the assay.
RESULTS: HLA-A*31:01 was confirmed to be significantly associated with definite/probable cases of CBZ-related SJS/TEN (p = 0.0040). Three single nucleotide polymorphisms, rs1150738, rs3869066 and rs259945, were in absolute linkage disequilibrium with HLA-A*31:01 in 210 Japanese SJS/TEN patients. Robust genotyping of rs3869066 in ZNRD1-AS1 was developed using polymerase chain reaction-restriction fragment length polymorphism assays.
CONCLUSION: Single nucleotide polymorphism genotyping is less time consuming and cheaper than conventional HLA typing, and would be useful for identifying Japanese patients at risk of CBZ-related SJS/TEN.
MATERIALS & METHODS: DNAs from Japanese SJS/TEN patients were used for genotyping and developing the assay.
RESULTS: HLA-A*31:01 was confirmed to be significantly associated with definite/probable cases of CBZ-related SJS/TEN (p = 0.0040). Three single nucleotide polymorphisms, rs1150738, rs3869066 and rs259945, were in absolute linkage disequilibrium with HLA-A*31:01 in 210 Japanese SJS/TEN patients. Robust genotyping of rs3869066 in ZNRD1-AS1 was developed using polymerase chain reaction-restriction fragment length polymorphism assays.
CONCLUSION: Single nucleotide polymorphism genotyping is less time consuming and cheaper than conventional HLA typing, and would be useful for identifying Japanese patients at risk of CBZ-related SJS/TEN.