Properties and use of mumps viral antigen for detection of specific IgG and IgM antibodies in enzyme-linked immunosorbent assay.

Specific mumps virus antigens of three purification degrees have been prepared and their quality was evaluated in the enzyme-linked immunosorbent assay (ELISA) with specific human sera. Two enzyme immunoassays were elaborated, namely a simple sandwich method for IgG and the IgM-capture technique for IgM detection. Four different lots of mumps antigen were tested in these two ELISA systems. All antigens of corresponding purification degrees showed practically the same properties.