Journal of cataract and refractive surgery · 2004
Evaluation of biofilm formation on nylon sutures removed from clinically noninfected eyes after cataract surgery.
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Pan James C H, Lau Boon-Harn, Fam Han-Bor, Ng Mary M L
Аннотация
PURPOSE: To determine the presence of bacterial biofilm on nylon sutures removed from clinically noninfected eyes after cataract surgery.
SETTING: The Eye Institute, Tan Tock Seng Hospital, Singapore, Singapore.
METHODS: Sutures were removed from 10 eyes after cataract surgery at different time periods. Immediately after removal, the sutures were fixed and dehydrated. All sutures were viewed by scanning electron microscopy, and 6 were also viewed by transmission electron microscopy (TEM).
RESULTS: There was no evidence of bacterial biofilm formation on the nylon sutures. Significant cellular debris was seen, mainly at the knots. Clusters of coccoid-shaped structures were visible; however, examination by TEM showed they were not bacteria.
CONCLUSION: There was no evidence of biofilm formation on sutures removed after cataract surgery from clinically noninfected eyes.
SETTING: The Eye Institute, Tan Tock Seng Hospital, Singapore, Singapore.
METHODS: Sutures were removed from 10 eyes after cataract surgery at different time periods. Immediately after removal, the sutures were fixed and dehydrated. All sutures were viewed by scanning electron microscopy, and 6 were also viewed by transmission electron microscopy (TEM).
RESULTS: There was no evidence of bacterial biofilm formation on the nylon sutures. Significant cellular debris was seen, mainly at the knots. Clusters of coccoid-shaped structures were visible; however, examination by TEM showed they were not bacteria.
CONCLUSION: There was no evidence of biofilm formation on sutures removed after cataract surgery from clinically noninfected eyes.