American journal of veterinary research · 2000
Methicillin-resistant coagulase-negative staphylococci isolated from healthy horses in Japan.
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Yasuda R, Kawano J, Onda H, Takagi M, Shimizu A, Anzai T
Аннотация
OBJECTIVE: To determine patterns of methicillin-resistant staphylococci isolated from apparently healthy horses.
SAMPLE POPULATION: 44 horses from 8 riding clubs in Japan.
PROCEDURE: Methicill in-resistant staphylococci were isolated from the skin or nares, using a selective medium containing a beta-(symboric) lactam antibiotic, ceftizoxime. Clonality of isolates was determined by use of pulsed-field gel electrophoresis. Detection of mecA, mecl, and mecR1 genes was accomplished by use of polymerase chain reactions.
RESULT: Of the 44 horses, 13 (29.5%) yielded 15 isolates of methicillin-resistant staphylococci. The 15 isolates were identified as 6 species (Staphylococcus epidermidis, S lentus, S saprophyticus, S xylosus, S sciuri, and S haemolyticus). However, methicillin-resistant S aureus was seldom isolated. Each isolate contained the mecA gene and had a high resistance to beta-lactam antibiotics. Some isolates also were resistant to other antibiotics such as erythromycin and kanamycin.
CONCLUSIONS AND CLINICAL RELEVANCE: Methicillin-resistant coagulase-negative staphylococci that were highly resistant to various antibiotics were isolated from apparently healthy horses in Japan. These organisms must be considered a potential threat to horses and veterinarians who care for them.
SAMPLE POPULATION: 44 horses from 8 riding clubs in Japan.
PROCEDURE: Methicill in-resistant staphylococci were isolated from the skin or nares, using a selective medium containing a beta-(symboric) lactam antibiotic, ceftizoxime. Clonality of isolates was determined by use of pulsed-field gel electrophoresis. Detection of mecA, mecl, and mecR1 genes was accomplished by use of polymerase chain reactions.
RESULT: Of the 44 horses, 13 (29.5%) yielded 15 isolates of methicillin-resistant staphylococci. The 15 isolates were identified as 6 species (Staphylococcus epidermidis, S lentus, S saprophyticus, S xylosus, S sciuri, and S haemolyticus). However, methicillin-resistant S aureus was seldom isolated. Each isolate contained the mecA gene and had a high resistance to beta-lactam antibiotics. Some isolates also were resistant to other antibiotics such as erythromycin and kanamycin.
CONCLUSIONS AND CLINICAL RELEVANCE: Methicillin-resistant coagulase-negative staphylococci that were highly resistant to various antibiotics were isolated from apparently healthy horses in Japan. These organisms must be considered a potential threat to horses and veterinarians who care for them.
Предметные рубрики MeSH
AnimalsBacterial ProteinsBacteriological TechniquesCarrier ProteinsCoagulaseDNA PrimersElectrophoresis, Agar GelElectrophoresis, Gel, Pulsed-FieldHexosyltransferasesHorsesJapanMethicillin ResistanceMicrobial Sensitivity TestsMuramoylpentapeptide CarboxypeptidasePenicillin-Binding ProteinsPeptidyl TransferasesPolymerase Chain ReactionRepressor ProteinsSkinStaphylococcus