Methods in molecular biology (Clifton, N.J.) · 2021
Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development.
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Chen Ting-Hsuan, Hong Jo-Yu, Liu Chia-Chyi, Chen Chung-Chu, Jan Jia-Tsrong, Wu Suh-Chin
Аннотация
Virus-like particle (VLP) technology is an alternative platform for developing vaccines to combat seasonal and pandemic influenza. Influenza VLPs are non-infectious nanoparticles that can elicit effective vaccine immunogenicity in hosts. B-cell-activating factor (BAFF, or BLyS) and a proliferation-inducing ligand (APRIL) are members of the tumor necrosis factor (TNF) superfamily of cytokines. Both BAFF and APRIL are homotrimers that interact with homotrimeric receptors. Here, we report a method of the production of influenza VLPs by molecular incorporation with BAFF or APRIL homotrimers to interact with their receptors. We engineered the VLPs by direct fusion of BAFF or APRIL to the transmembrane anchored domain of the hemagglutinin (HA) gene. We also describe procedures for the production of BAFF-VLPs containing H5H7 and H1H5H7 for multi-subtype vaccine development.
Предметные рубрики MeSH
AnimalsAntigens, ViralB-Cell Activating FactorBaculoviridaeCloning, MolecularGene ExpressionHemagglutination TestsHemagglutinin Glycoproteins, Influenza VirusHumansInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesPlasmidsRecombinant Fusion ProteinsTumor Necrosis Factor Ligand Superfamily Member 13Vaccines, Virus-Like Particle