Ultra short waves alleviate ulcerative colitis via TRPV2 mediated-macrophage polarization.

Ultra short wave (USW) therapy, known for its deep tissue penetration in electromagnetic waves, is clinically used to treat local inflammation and alleviate pain, yet its role in intestinal inflammation via macrophage regulation remains unclear. We investigated whether USW exerts non-thermal effects on macrophages to alleviate colitis using dextran sulfate sodium (DSS)-induced mouse models and LPS-stimulated BMDMs/THP-1 cells. Results demonstrated that USW significantly ameliorated intestinal inflammation by directly targeting macrophages, as macrophage depletion completely abrogated its therapeutic effects. Mechanistically, USW induced distinct shifts in intracellular calcium flux and modulated cytokine expression through the TRPV2/CaMKII/Nrf2 signaling pathway. This study confirms that USW alleviates colitis by regulating macrophage polarization and calcium signaling, uncovering a distinct therapeutic pathway and highlighting the potential of non-invasive USW treatment as an effective strategy for improving colitis prognosis.