Enzyme-linked immunosorbent assay (ELISA) of antibodies to eight common neurotropic viruses.

Enzyme-linked immunosorbent assay (ELISA) methods were developed for determining serum levels of antibodies to adenovirus, cytomegalovirus, Epstein-Barr virus, herpes simplex type I virus, measles virus, mumps virus, rubella virus, and varicella zoster virus. The assays were evaluated for specificity, range, precision and accuracy and found to give satisfactory performances. Serum samples from 76 blood donors were assayed for the different antibodies. The antibodies to adenovirus and the members of the Herpesviridae had wide concentration ranges, with a significant minority of the samples having zero or very low levels, whereas the antibodies to measles, mumps and rubella had narrower distributions, with very few samples having levels below the detection limit of the ELISA.