Contact dermatitis · 2013
Characterization of the T cell response in allergic contact dermatitis caused by corticosteroids.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Baeck Marie, Soria Angèle, Marot Liliane, Theate Ivan, Hendrickx Emilie, Van Belle Astrid, Goossens An, Tennstedt Dominique, Dachelet Claire, Jaeger Johnathan, Pilette Charles, Renauld Jean-Christophe, Van Baren Nicolas, Rozières Aurore, Nicolas Jean-Fançois, Dumoutier Laure
Аннотация
BACKGROUND: Delayed allergic hypersensitivity reactions have classically been described as type IV reactions, which are caused by T cells; however, the respective roles of CD4(+) and CD8(+) cells are yet to be defined. A central role for CD8(+) cytotoxic T cells as effector cells has been suggested.
OBJECTIVES: To determine the type of T cell involved in corticosteroid allergy.
METHODS: We analysed the kinetics of T cell recruitment and the cytokine production profile in positive patch tests of 27 corticosteroid-sensitized patients, as compared with control sites and control subjects. Skin biopsies, collected at 8, 24 and 48 hr following drug application, were embedded in paraffin for histological and immunohistological staining, and, in some cases, also deep-frozen for gene expression analyses.
RESULTS: CD3(+) T cells were rapidly recruited in concert with the positivity of the patch test sites. High levels of interleukin (IL)-4, IL-5 and, to a lesser extent, interferon-γ suggested that both Th2 and Th1 cytokines were implicated. IL-4 was also produced by γδ T cell receptor (TCR) lymphocytes.
CONCLUSIONS: This study showed that, in allergic contact dermatitis caused by corticosteroids, the inflammatory infiltrate is composed of CD3(+) T cells with a predominant Th2 cytokine profile, among which IL-4 is also produced by γδ TCR lymphocytes.
OBJECTIVES: To determine the type of T cell involved in corticosteroid allergy.
METHODS: We analysed the kinetics of T cell recruitment and the cytokine production profile in positive patch tests of 27 corticosteroid-sensitized patients, as compared with control sites and control subjects. Skin biopsies, collected at 8, 24 and 48 hr following drug application, were embedded in paraffin for histological and immunohistological staining, and, in some cases, also deep-frozen for gene expression analyses.
RESULTS: CD3(+) T cells were rapidly recruited in concert with the positivity of the patch test sites. High levels of interleukin (IL)-4, IL-5 and, to a lesser extent, interferon-γ suggested that both Th2 and Th1 cytokines were implicated. IL-4 was also produced by γδ T cell receptor (TCR) lymphocytes.
CONCLUSIONS: This study showed that, in allergic contact dermatitis caused by corticosteroids, the inflammatory infiltrate is composed of CD3(+) T cells with a predominant Th2 cytokine profile, among which IL-4 is also produced by γδ TCR lymphocytes.
Предметные рубрики MeSH
AdultAgedBiomarkersBiopsyBudesonideCD3 ComplexCase-Control StudiesDermatitis, Allergic ContactDrug EruptionsFemaleFlow CytometryGlucocorticoidsHumansHydrocortisoneImmunohistochemistryInterferon-gammaInterleukin-4Interleukin-5Linear ModelsLogistic ModelsMaleMiddle AgedPatch TestsReceptors, Antigen, T-Cell, gamma-deltaReverse Transcriptase Polymerase Chain ReactionSkinT-Lymphocytes