Arthritis and rheumatism · 1993
Spontaneous production of Epstein-Barr virus by B lymphoblastoid cell lines obtained from patients with Sjögren's syndrome. Possible involvement of a novel strain of Epstein-Barr virus in disease pathogenesis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Tateishi M, Saito I, Yamamoto K, Miyasaka N
Аннотация
OBJECTIVE: To investigate the involvement of Epstein-Barr virus (EBV) in the pathogenesis of Sjögren's syndrome (SS) and to examine whether the spontaneous production of EBV is unique to SS B cell lines.
METHODS: B cell lines were established from peripheral blood mononuclear cells (PBMC) of patients with systemic lupus erythematosus, rheumatoid arthritis, and SS. The cord blood immortalization assay, flow cytometric analysis, and polymerase chain reaction (PCR) were used to detect EBV production by B cell lines.
RESULTS: SS B cell lines produced EBV at a higher frequency, and in significantly larger amounts, than did other B cell lines. However, no correlation with the amount of EBV DNA in the genome of B cell lines was found. PCR analysis revealed that EBV with a B95-8--like U2 region was dominant in SS B cell lines.
CONCLUSION: Spontaneous, massive production of EBV by B cell lines is unique to SS, and may contribute to the polyclonal B cell activation seen in this disease.
METHODS: B cell lines were established from peripheral blood mononuclear cells (PBMC) of patients with systemic lupus erythematosus, rheumatoid arthritis, and SS. The cord blood immortalization assay, flow cytometric analysis, and polymerase chain reaction (PCR) were used to detect EBV production by B cell lines.
RESULTS: SS B cell lines produced EBV at a higher frequency, and in significantly larger amounts, than did other B cell lines. However, no correlation with the amount of EBV DNA in the genome of B cell lines was found. PCR analysis revealed that EBV with a B95-8--like U2 region was dominant in SS B cell lines.
CONCLUSION: Spontaneous, massive production of EBV by B cell lines is unique to SS, and may contribute to the polyclonal B cell activation seen in this disease.
Предметные рубрики MeSH
AdultAgedAntigens, ViralArthritis, RheumatoidB-LymphocytesBase SequenceBlotting, SouthernCapsidCell LineCell Transformation, ViralCytokinesDNA, ViralEnzyme-Linked Immunosorbent AssayFemaleGene AmplificationHerpesvirus 4, HumanHumansInterleukin-1Lupus Erythematosus, SystemicMiddle AgedPolymerase Chain ReactionSalivary GlandsSjogren's Syndrome