Ultracentrifugation for Coagulation Testing.

Lipemia is known to potentially affect coagulation testing. It may be detected with newer coagulation analyzers that are validated to assess hemolysis, icterus, and lipemia (HIL) in a plasma sample. In samples with lipemia where accuracy of the test result is compromised, strategies for mitigating the lipemia interferences would be required. The tests affected by lipemia are those using chronometric, chromogenic, immunologic, or other light scattering/reading principles. Ultracentrifugation is one process that has been effectively demonstrated to remove lipemia from blood samples to allow for more accurate measurements. In this chapter, a description of one ultracentrifugation method is provided.