Evaluation of ELISA, IFAT and trichinoscopy in experimental trichinosis: comparing larval antigens prepared from rat and pig muscles.

Two antigens were prepared, the first one was crude larval antigens derived from pigs and rats. The two antigens were tested by the enzyme linked immunosorbent assay (ELISA). The 2nd one, was two cuticular larval antigens derived from pigs and rats and were tested by the indirect immunofluorescent antibody test (IFAT). Both tests were used to evaluate the sensitivity and specificity of these different antigens. Serum samples from infected rats and pigs were tested with different antigens by ELISA and IFAT. At the end of experiment, all animals were killed and use trichinoscopy to reveal sensitivity and specificity versus ELISA and IFAT.