Journal of the Egyptian Society of Parasitology · 1994
Humoral and cellular immune responses in experimental trichinosis.
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abou Hassan M M, Seleem M A, Etewa S E
Аннотация
This work was designed to study the relation between cellular and humoral immune responses in experimentally infected albino rats with T. spiralis larvae over a period of four weeks. Cell-mediated immune response (measured by lymphoblast transformation test) revealed a marked lymphoblast response during the intestinal phase, but the reaction became irregular with larval encystation in muscles. Humoral immune response was estimated by both of micro-and Dot-ELISA techniques. It was obvious that significant higher O.D. readings and dot color intensities were observed in the muscular phase than in intestinal phase. The results proved that micro-ELISA had the least accuracy (66.6%) and poorest specificity (81.25%), while Dot-ELISA showed the best sensitivity (100%) and highest specificity (87.5%). Lymphoblast transformation was as specific as Dot-ELISA.