[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 1999
[An experimental investigation of inhibition of interferon on lens epithelial cell growth in vitro].
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Hu N, Qiu X, Gong Q, Guan H
Аннотация
OBJECTIVE: To investigate the inhibition of alpha-interferon (alpha-IFN) and gamma-interferon (gamma-IFN) on rabbit lens epithelial cell (RLEC) proliferation in vitro and their effective concentrations.
METHODS: The second and third passage of RLEC were used for assay. Various concentrations of alpha-IFN or gamma-IFN were added into the culture medium and RLECs were exposed to these drugs. After 24 and 72 hours, the inhibition of RLECs was determined by counting the RLEC numbers on a counting plate and MTT colorimetric assay.
RESULTS: Alpha-IFN and gamma-IFN may reduce the proliferation of RLECs at the concentration of 10(3) - 10(4) IU/ml. The inhibition of gamma-IFN was a little stronger than that of alpha-IFN.
CONCLUSION: The experiment provides a scientific basis for selection of drugs to prevent after cataract.
METHODS: The second and third passage of RLEC were used for assay. Various concentrations of alpha-IFN or gamma-IFN were added into the culture medium and RLECs were exposed to these drugs. After 24 and 72 hours, the inhibition of RLECs was determined by counting the RLEC numbers on a counting plate and MTT colorimetric assay.
RESULTS: Alpha-IFN and gamma-IFN may reduce the proliferation of RLECs at the concentration of 10(3) - 10(4) IU/ml. The inhibition of gamma-IFN was a little stronger than that of alpha-IFN.
CONCLUSION: The experiment provides a scientific basis for selection of drugs to prevent after cataract.