Asian Pacific journal of cancer prevention : APJCP · 2012
Antifibrotic effect of curcumin in TGF-β 1-induced myofibroblasts from human oral mucosa.
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Zhang Shan-Shan, Gong Zhao-Jian, Li Wen-Hui, Wang Xiao, Ling Tian-You
Аннотация
BACKGROUND: Myofibroblasts play an important role in the development of oral submucous fibrosis (OSF). In the current study, we investigate the effect of curcumin on growth and apoptosis of myofibroblasts derived from human oral mucosa.
METHODS: Myofibroblasts were generated by incubating fibroblasts, obtained from human oral mucosa, with transforming growth factor-β 1 (TGF-β 1). MTT, PI staining, and FACS assays were used to investigate curcumin's effect on proliferation and cell cycle of fibroblasts and myofibroblasts. Annexin V/PI binding and FACS assays were used to examine apoptosis of myofibroblasts, Western blotting to determine the levels of Bcl-2 and Bax, and enzyme-linked immunosorbant assay was employed to examine the levels of collagen type I and III in the supernatants of myofibroblasts.
RESULTS: Curcumin inhibits proliferation of fibroblasts and myofibroblasts; it also disturbs the cell cycle, induces apoptosis and decreases the generation of collagen type I and III in myofibroblasts, which are more sensitive to its effects than fibroblasts. Curcumin induces apoptosis in myofibroblasts by down-regulating the Bcl-2/ Bax ratio.
CONCLUSION: Our results demonstrate the antifibrotic effect of curcumin in vitro. It may therefore be a candidate for the treatment of OSF.
METHODS: Myofibroblasts were generated by incubating fibroblasts, obtained from human oral mucosa, with transforming growth factor-β 1 (TGF-β 1). MTT, PI staining, and FACS assays were used to investigate curcumin's effect on proliferation and cell cycle of fibroblasts and myofibroblasts. Annexin V/PI binding and FACS assays were used to examine apoptosis of myofibroblasts, Western blotting to determine the levels of Bcl-2 and Bax, and enzyme-linked immunosorbant assay was employed to examine the levels of collagen type I and III in the supernatants of myofibroblasts.
RESULTS: Curcumin inhibits proliferation of fibroblasts and myofibroblasts; it also disturbs the cell cycle, induces apoptosis and decreases the generation of collagen type I and III in myofibroblasts, which are more sensitive to its effects than fibroblasts. Curcumin induces apoptosis in myofibroblasts by down-regulating the Bcl-2/ Bax ratio.
CONCLUSION: Our results demonstrate the antifibrotic effect of curcumin in vitro. It may therefore be a candidate for the treatment of OSF.
Предметные рубрики MeSH
Anti-Inflammatory Agents, Non-SteroidalApoptosisBlotting, WesternCell CycleCell DifferentiationCell ProliferationCells, CulturedChild, PreschoolCollagen Type ICurcuminEnzyme-Linked Immunosorbent AssayFibroblastsFlow CytometryHumansImmunoenzyme TechniquesInfantMouth MucosaMyofibroblastsOral Submucous FibrosisProto-Oncogene Proteins c-bcl-2Signal TransductionTransforming Growth Factor beta1bcl-2-Associated X Protein