Propagation of large quantities of poliovirus in human lymphoblastoid cells grown in a serum-free medium.

All three types of poliovirus replicated to good titers in Epstein-Barr virus-transformed human lymphoblastoid cells isolated from patients with infectious mononucleosis. Cells were grown in a serum-free medium, which consisted of enriched Eagle's medium with addition of 0.1% albumin, 0.1% Intralipid and 1 mg/l of transferrin. Large quantities of virus could be produced in 2- to 20-l roller flasks or in spinner flasks. The propagated poliovirus gave an antibody response that complies with requirements for vaccine production.