American journal of rhinology & allergy · 2012
Suppression of connexin 26 is related to protease-activated receptor 2-mediated pathway in patients with allergic rhinitis.
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Zheng Jing, Liu Wenlong, Fan Yunping, Ye Xingling, Xia Wentong, Wang Hongtian, Shi Jianbo, Xu Geng, Li Huabin
Аннотация
BACKGROUND: Connexin (Cx) 26 plays a key role in maintaining the integrity of tight junctions. However, the expression and modulation of Cx26 in allergic rhinitis (AR) has not been well understood.
METHODS: We detected the expression of Cx26 in house-dust mite (HDM)-sensitized AR patients and investigated the Cx26 production and modulation in primary human nasal epithelial cells (HNECs) and BEAS-2B cells after treatment with the allergen Der p 1 from Dermatophagoides pteronyssinus.
RESULTS: We found that the mRNA and protein levels of Cx26 were significantly down-regulated in AR patients compared with the control. Der p 1 was found to induce protease-activated receptor 2 (PAR2) expression and suppress Cx26 production significantly in vitro. PAR2 siRNA was shown to prevent the suppression of Cx26 induced by Der p 1 in BEAS-2B cells.
CONCLUSION: The suppression of Cx26 in HDM-sensitized AR patients is related to a PAR2-mediated pathway and might serve during the initiation and maintenance of AR. Targeting the PAR2-mediated Cx26 suppression may be a potential means of preventing allergic sensitization.
METHODS: We detected the expression of Cx26 in house-dust mite (HDM)-sensitized AR patients and investigated the Cx26 production and modulation in primary human nasal epithelial cells (HNECs) and BEAS-2B cells after treatment with the allergen Der p 1 from Dermatophagoides pteronyssinus.
RESULTS: We found that the mRNA and protein levels of Cx26 were significantly down-regulated in AR patients compared with the control. Der p 1 was found to induce protease-activated receptor 2 (PAR2) expression and suppress Cx26 production significantly in vitro. PAR2 siRNA was shown to prevent the suppression of Cx26 induced by Der p 1 in BEAS-2B cells.
CONCLUSION: The suppression of Cx26 in HDM-sensitized AR patients is related to a PAR2-mediated pathway and might serve during the initiation and maintenance of AR. Targeting the PAR2-mediated Cx26 suppression may be a potential means of preventing allergic sensitization.
Предметные рубрики MeSH
AdultAnimalsAntigens, DermatophagoidesArthropod ProteinsCells, CulturedConnexin 26ConnexinsCysteine EndopeptidasesFemaleGene Expression RegulationHumansMaleMiddle AgedNasal MucosaNasal PolypsPyroglyphidaeRNA, Small InterferingReceptor, PAR-2Rhinitis, Allergic, PerennialSignal TransductionYoung Adult