Three-dimensional super-resolution imaging of whole cells using a high numerical aperture oblique-plane microscope.

We present HOPE-STORM, a high-numerical-aperture oblique-plane microscope enabling whole-cell super-resolution imaging with an effective NA of 1.40. The system is compatible with DNA-PAINT, resolving nuclear pore complex (NPC) structures at 7.5 nm. We demonstrate dual-color super-resolution imaging of intact cells and, for the first time, to our knowledge, quantify the 3D organization of dynamin-related protein 1 (DRP1) complexes at mitochondrial fission sites. Our findings provide structural insights into DRP1 dynamics and mitochondrial fission mechanisms.