Cornea · 2018
Femtosecond Laser-Assisted Small Incision Endokeratophakia Using a Xenogeneic Lenticule in Rhesus Monkeys.
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He Miao, Jin He, He Hong, Ding Hui, Wang Wei, Liu Liangping, Zhang Chi, Zhong Xingwu
Аннотация
PURPOSE: To evaluate the feasibility and safety of xenogeneic endokeratophakia in rhesus monkeys and to explore the effects of a concave lenticule on refractive power.
METHODS: Three adult New Zealand white rabbits and 6 healthy rhesus monkeys were used. The xenogenic concave grafts were created from the rabbits using a modified small incision lenticule extraction technique; after being cryopreserved in glycerol for 1 week, the grafts were implanted into the monkey recipient corneas. Spherical equivalent (SE), central corneal thickness, and keratometry curvature were assessed preoperatively, 1 week, 1, 4 and 6 months postoperatively. The quality of the xenogenic graft was also assessed by slit-lamp microscopy, in vivo confocal microscopy, and optical coherence tomography with anterior segment imaging.
RESULTS: The graft appeared to be swollen a day after the operation but reduced considerably after a week. A trend of a lower refractive power (hyperopic shift) was demonstrated in relation to the SE after concave graft implantation. The mean SE increased from -0.60 ± 1.31 (median -0.69, interquartile range -1.00 to 0.50) preoperatively to 0.75 ± 1.27 (median 1.38, interquartile range -0.25 to 1.63) at 1 month postoperatively (P = 0.01). Central corneal thickness was significantly thicker each time after surgery compared with that recorded preoperatively (P < 0.01). The anterior and posterior interface between the graft and stroma was visible during the study. Corneal nerve regeneration was evident at 6 months postoperatively. The xenogeneic concave graft was stable and transparent at follow-up. Severe adverse events or evidence of a rejection response were not observed.
CONCLUSIONS: Femtosecond laser-assisted small incision endokeratophakia using a xenogeneic corneal lenticule seems to be feasible and safe, which may provide a new method for myopia correction and keratoconus treatment.
METHODS: Three adult New Zealand white rabbits and 6 healthy rhesus monkeys were used. The xenogenic concave grafts were created from the rabbits using a modified small incision lenticule extraction technique; after being cryopreserved in glycerol for 1 week, the grafts were implanted into the monkey recipient corneas. Spherical equivalent (SE), central corneal thickness, and keratometry curvature were assessed preoperatively, 1 week, 1, 4 and 6 months postoperatively. The quality of the xenogenic graft was also assessed by slit-lamp microscopy, in vivo confocal microscopy, and optical coherence tomography with anterior segment imaging.
RESULTS: The graft appeared to be swollen a day after the operation but reduced considerably after a week. A trend of a lower refractive power (hyperopic shift) was demonstrated in relation to the SE after concave graft implantation. The mean SE increased from -0.60 ± 1.31 (median -0.69, interquartile range -1.00 to 0.50) preoperatively to 0.75 ± 1.27 (median 1.38, interquartile range -0.25 to 1.63) at 1 month postoperatively (P = 0.01). Central corneal thickness was significantly thicker each time after surgery compared with that recorded preoperatively (P < 0.01). The anterior and posterior interface between the graft and stroma was visible during the study. Corneal nerve regeneration was evident at 6 months postoperatively. The xenogeneic concave graft was stable and transparent at follow-up. Severe adverse events or evidence of a rejection response were not observed.
CONCLUSIONS: Femtosecond laser-assisted small incision endokeratophakia using a xenogeneic corneal lenticule seems to be feasible and safe, which may provide a new method for myopia correction and keratoconus treatment.