Alternative therapies in health and medicine · 2024
Preliminary Screening and Study of Key Gene Expression in Endometrial Window Period.
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Zhou Shu, Wang Nan, Zhang Lixing, Chen Huan, Liu Xiu, Xu Cen, Yang Yu, Wang Lin, Yuan Donglan, Qian Hua, Ge Hongshan
Аннотация
BACKGROUND: Implantation is a highly coordinated event involving both embryonic and endometrial participation. The endometrium expresses a complex array of proteins during the menstrual cycle many of which help to define a period of receptivity collectively known as the "window of implantation."
OBJECTIVE: Using high-throughput RNA sequencing technology analysis to find differentially expressed genes before and after the endometrial window, and search for key marker genes of the membrane implantation window.
DESIGN: This was a retrospective study.
SETTING: This study was performed in the Department of Obstetrics and Gynecology, Taizhou People's Hospital.
PARTICIPANTS: Fifty patients with repeated implantation failure in in vitro fertilization were selected and were divided into (1) the normal window group (36 cases); (2) the window forward group (8 cases); and (3) the window backward group (6 cases) based on endometrial biopsy findings.
INTERVENTIONS: Using RNA sequencing technology combined with biological information analysis tools to analyze the differentially-expressed genes in 9 samples. Gene Ontology databases were used for the functional annotation of these differentially-expressed genes. Kyoto Encyclopedia of Genes and Genomes analysis was used to draw a signal path diagram.
PRIMARY OUTCOME MEASURES: (1) Screening of differentially-expressed genes and (2) functional analysis of the differential genes.
RESULTS: A total of 22 differentially-expressed genes related to endometrial receptivity were obtained by transcriptome sequencing. Seven of the 22 differentially-expressed genes have been shown to have a close relationship with the endometrial receptive window period. Further, it was proved that the Wnt signaling pathway and mitogen-activated protein kinase signaling pathway were closely related to endometrial receptivity.
CONCLUSIONS: The present study identified a series of key genes and pathways that may be involved in the endometrial window period, providing an experimental and theoretical basis for exploring the personalized embryo transfer program.
OBJECTIVE: Using high-throughput RNA sequencing technology analysis to find differentially expressed genes before and after the endometrial window, and search for key marker genes of the membrane implantation window.
DESIGN: This was a retrospective study.
SETTING: This study was performed in the Department of Obstetrics and Gynecology, Taizhou People's Hospital.
PARTICIPANTS: Fifty patients with repeated implantation failure in in vitro fertilization were selected and were divided into (1) the normal window group (36 cases); (2) the window forward group (8 cases); and (3) the window backward group (6 cases) based on endometrial biopsy findings.
INTERVENTIONS: Using RNA sequencing technology combined with biological information analysis tools to analyze the differentially-expressed genes in 9 samples. Gene Ontology databases were used for the functional annotation of these differentially-expressed genes. Kyoto Encyclopedia of Genes and Genomes analysis was used to draw a signal path diagram.
PRIMARY OUTCOME MEASURES: (1) Screening of differentially-expressed genes and (2) functional analysis of the differential genes.
RESULTS: A total of 22 differentially-expressed genes related to endometrial receptivity were obtained by transcriptome sequencing. Seven of the 22 differentially-expressed genes have been shown to have a close relationship with the endometrial receptive window period. Further, it was proved that the Wnt signaling pathway and mitogen-activated protein kinase signaling pathway were closely related to endometrial receptivity.
CONCLUSIONS: The present study identified a series of key genes and pathways that may be involved in the endometrial window period, providing an experimental and theoretical basis for exploring the personalized embryo transfer program.