[The significance of the expression of matrix metalloproteinases in the differential diagnosis of periodontal diseases].

UNLABELLED: Investigation of features of expression of matrix metalloproteinases (MMPs) is important for both understanding the mechanisms of the pathogenesis of periodontal diseases, and determining the nature of their course in order to choose a correct and timely treatment strategy.

OBJECTIVE

To establish the value of MMPs for the diagnosis and determination of the nature of the course of periodontitis at the stage of disease manifestation, by morphometrically assessing the expression of MMPs in the gingival biopsy material.

MATERIAL AND

METHODS

Gingival biopsy specimens from 82 patients with rapidly progressing (n=26), chronic simple (n=18), and chronic complex (n=38) periodontitis were analyzed. Morphometric and statistical analysis of the expression of MMPs was carried out using Aperio ImageScope v. 12.4.0.5043, Statistica 10.0, and MedCalc (p<0.05).

RESULTS

Analysis of the nature of MMP expression in the gingival biopsy material of patients with periodontal diseases showed that MMP2 and MMP13 were approximately equally involved in the development and course of all the studied forms of periodontitis. An increase in the expression of MMP1, MMP8, and MMP14 and a decrease in that of MMP9 are of the greatest importance in the pathogenesis of a rapidly progressing process. The performed ROC analysis confirmed the significance of the parameters of general and stromal expression of MMP1, MMP9, and MMP14, and mainly stromal expression of MMP8 for the differential diagnosis of rapidly progressing periodontitis with chronic forms, including chronic complex periodontitis.

CONCLUSION

The expression indicators of MMR1, MMR8, MMR9, and MMR14 are most informative in determining the course of periodontitis at the stage of disease manifestation and differential diagnosis of rapidly progressing periodontitis with chronic simplex and complex periodontitis.