Journal of global antimicrobial resistance · 2018
Pandemic Escherichia coli ST648 isolate harbouring fosA3 and blaCTX-M-8 on an IncI1/ST113 plasmid: A new successful combination for the spread of fosfomycin resistance?
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Dantas Palmeira Josman, Ferreira Helena, Madec Jean-Yves, Haenni Marisa
Аннотация
OBJECTIVES: The emergence of Enterobacteriaceae isolates resistant to the last-resort antibiotic fosfomycin outside of Asia is a public-health issue. Here we report the draft genome of an Escherichia coli isolate presenting both an extended-spectrum β-lactamase (ESBL) and the fosA3 gene in a healthy cow in Brazil.
METHODS: Whole genomic DNA from E. coli E12 was extracted and 2×150-bp paired-end reads were generated using Illumina sequencing technology. De novo genome assembly was performed using SPAdes v.3.11 and the draft genome was annotated by the NCBI Prokaryotic Genome Annotation Pipeline. Further analyses were performed using the Center for Genomic Epidemiology databases.
RESULTS: The 5045934-bp genome displayed several resistance genes, including the fosA3 and blaCTX-M-8 genes. Southern blot experiments showed that they were co-located on an IncI1/ST113 plasmid.
CONCLUSION: Presence of the fosA3 gene on the same common plasmid as blaCTX-M-8 will have to be monitored. This draft genome provides data that will help in tracing the dissemination of this gene and the evolution of its plasmidic support.
METHODS: Whole genomic DNA from E. coli E12 was extracted and 2×150-bp paired-end reads were generated using Illumina sequencing technology. De novo genome assembly was performed using SPAdes v.3.11 and the draft genome was annotated by the NCBI Prokaryotic Genome Annotation Pipeline. Further analyses were performed using the Center for Genomic Epidemiology databases.
RESULTS: The 5045934-bp genome displayed several resistance genes, including the fosA3 and blaCTX-M-8 genes. Southern blot experiments showed that they were co-located on an IncI1/ST113 plasmid.
CONCLUSION: Presence of the fosA3 gene on the same common plasmid as blaCTX-M-8 will have to be monitored. This draft genome provides data that will help in tracing the dissemination of this gene and the evolution of its plasmidic support.