Oral diseases · 2020
Assessment of mucin and alpha-amylase levels in gingival crevicular fluid of chronic periodontitis patients.
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Vadiati Saberi Bardia, Yousefi Farouq, Yousefi Tooba, Qujeq Durdi
Аннотация
BACKGROUND: Chronic periodontitis is the most common form of periodontitis. Several immune and inflammatory factors responsible for periodontal destruction have been found in gingival crevicular fluid (GCF). The current study was conducted to determine the correlation between mucin and alpha-amylase protein values in GCF with chronic periodontitis.
METHOD: Forty-five patients with moderate-to-severe chronic periodontitis were selected. Samples of GCF were taken from a specific part of a single root tooth and placed in a closed test tube containing phosphate-buffered saline (PBS) (pH = 7). Sampling was done again after one month. Pre- and post-treatment samples were analyzed for measuring the levels of mucin and alpha-amylase proteins.
RESULTS: Paired t test results for these two variables showed that the difference between mucin and alpha-amylase levels before and after treatment is significant.
CONCLUSION: The level of both mucin and alpha-amylase in GCF in patients with chronic periodontitis was higher than that of patients who have recovered successfully; and evaluating the values of these two markers could be used to determine the activity of the periodontal disease.
METHOD: Forty-five patients with moderate-to-severe chronic periodontitis were selected. Samples of GCF were taken from a specific part of a single root tooth and placed in a closed test tube containing phosphate-buffered saline (PBS) (pH = 7). Sampling was done again after one month. Pre- and post-treatment samples were analyzed for measuring the levels of mucin and alpha-amylase proteins.
RESULTS: Paired t test results for these two variables showed that the difference between mucin and alpha-amylase levels before and after treatment is significant.
CONCLUSION: The level of both mucin and alpha-amylase in GCF in patients with chronic periodontitis was higher than that of patients who have recovered successfully; and evaluating the values of these two markers could be used to determine the activity of the periodontal disease.