Zhongguo shi yan xue ye xue za zhi · 2018
[Effect of IKK2 Inhibitor LY2409881 on Diffuse Large B-Cell Lymphoma Cell Apoptosis and Its Mechanism].
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Yang Pu, Gao Xiang, Jiao Jun, Xu Lin-Yan, Sang Wei, Sun Xiao-Kun, Xu Kai-Lin
Аннотация
OBJECTIVE: To investigate the effects and its potential mechanism of IKK2 inhibitor LY2409881 on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.
METHODS: CCK8 assay was used to detect the effect of LY2409881 on proliferation of DLBCL cell lines; Flow cytometry was used to analyze cell cycle; Western blot was used to analyze apoptosis and its potential mechanism.
RESULTS: LY2409881 inhibited the proliferation of multiple DLBCL cell lines obviously, and caused G1 cell arrest. Furtherly, LY2409881 inhibited the expression of c-FLIP, induced the activation of DR4 and caspase 8. Meanwhile, LY2409881 induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1 and BCL-2.
CONCLUSION: LY2409881 inhibits the proliferation of DLBCL cell lines, causes G1 cell arrest and induces apoptosis via the endogenous and exogenous apoptotic pathways.
METHODS: CCK8 assay was used to detect the effect of LY2409881 on proliferation of DLBCL cell lines; Flow cytometry was used to analyze cell cycle; Western blot was used to analyze apoptosis and its potential mechanism.
RESULTS: LY2409881 inhibited the proliferation of multiple DLBCL cell lines obviously, and caused G1 cell arrest. Furtherly, LY2409881 inhibited the expression of c-FLIP, induced the activation of DR4 and caspase 8. Meanwhile, LY2409881 induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1 and BCL-2.
CONCLUSION: LY2409881 inhibits the proliferation of DLBCL cell lines, causes G1 cell arrest and induces apoptosis via the endogenous and exogenous apoptotic pathways.