Journal of medical microbiology · 2002
Dihydrofolate reductase gene polymorphisms in Pneumocystis carinii f. sp. hominis in Japan.
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Takahashi Takashi, Endo Tokiomi, Nakamura Tetsuya, Sakashita Hiroyuki, Kimura Kyoko, Ohnishi Kenji, Kitamura Yoshihiro, Iwamoto Aikichi
Аннотация
This study examined polymorphisms in the dihydrofolate reductase (DHFR) gene of Pneumocystis carinii isolates from 27 patients with P. carinii pneumonia (PCP) in Japan. Four substitution sites with two synonymous and two non-synonymous changes were found. Two synonymous substitutions at nucleotide positions 540 and 312 were identified in one and 13 patients, respectively. Two amino acid substitutions (Ala67Val, Cysl66Tyr) were found in two different patients. No linkage of amino acid substitutions in DHFR to those in dihydropteroate synthase was observed. The two patients whose isolates showed non-synonymous DHFR mutations were not exposed to DHFR inhibitors before they developed PCP and were treated successfully with co-trimoxazole.
Предметные рубрики MeSH
Amino Acid SequenceAnti-Infective AgentsBase SequenceBronchoalveolar Lavage FluidDihydropteroate SynthaseGene AmplificationGenes, FungalHumansImmunocompromised HostJapanMolecular Sequence DataMutationPneumocystisPneumonia, PneumocystisPolymerase Chain ReactionPolymorphism, GeneticSequence AlignmentSequence AnalysisTetrahydrofolate DehydrogenaseTreatment FailureTrimethoprim, Sulfamethoxazole Drug Combination