Zhongguo yi miao he mian yi · 2010
[Optimization of method of titration rabies virus with fluorescence focus units assay].
Оценочное исследованиеСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Chai Miao, Xu Ge-lin, Sun Wen
Аннотация
OBJECTIVE: A new Fluorescence Focus Units (FFU) developed for Rabies Virus titration replace current reference method (LD50) so as to reduce the cost.
METHODS: The BSR cell was infected by triple serial dilution of the Rabies Virus CTN strain, then detect the fluorescent focus by FITC labeling anti-Rabies Virus monoclonal antibodies. All the samples were assayed in both FFU and LD50.
RESULTS: Data generated indicateed that a significant correlation between the two methods (r=99).
CONCLUSION: This method is simple and rapid. It will be considered as a useful alternative method to the LD50.
METHODS: The BSR cell was infected by triple serial dilution of the Rabies Virus CTN strain, then detect the fluorescent focus by FITC labeling anti-Rabies Virus monoclonal antibodies. All the samples were assayed in both FFU and LD50.
RESULTS: Data generated indicateed that a significant correlation between the two methods (r=99).
CONCLUSION: This method is simple and rapid. It will be considered as a useful alternative method to the LD50.