Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi · 2010
[The establishment of a rapid fluorescent focus inhibition test for testing rabies virus neutralizing antibody].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Yu Peng-cheng, Lv Xin-jun, Shen Xin-xin, Cao Lei, Zheng Xin-xiong, Shan Hu, Tang Qing
Аннотация
OBJECTIVE: To establish a rapid fluorescent inhibition test (RFFIT) for testing rabies virus neutralizing antibody and the titer of rabies virus neutralizing antibody. CVS-11 was used as the standard challenge virus, and three generations prepared for the establishment of the virus library.
METHODS: International standard for rabies immunoglobulin was used as the reference serum. RFFIT test was established under consulting the protocol of Institute of Pasteur, and its specificity, stability and reproducibility were validated.
RESULTS: We established the RFFIT which showed both good specificity (100%) and reproducibility (P > 0.5).
CONCLUSION: The establishment of RFFIT test perfected the rabies laboratory techniques and would enhance the overall ability in detecting rabies in China.
METHODS: International standard for rabies immunoglobulin was used as the reference serum. RFFIT test was established under consulting the protocol of Institute of Pasteur, and its specificity, stability and reproducibility were validated.
RESULTS: We established the RFFIT which showed both good specificity (100%) and reproducibility (P > 0.5).
CONCLUSION: The establishment of RFFIT test perfected the rabies laboratory techniques and would enhance the overall ability in detecting rabies in China.