Journal of global antimicrobial resistance · 2025
First identification of a multidrug-resistant Pseudomonas kurunegalensis clinical isolate co-carrying blaAFM-1 and blaIMP-1 in China.
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Fan Shuwei, Gong Yuzhuo, Chen Chunxiao, Shi Jingchao, Wu Cong
Аннотация
OBJECTIVES: The objective of this study was to elucidate the genetic context and characterize chromosome co-carrying blaAFM-1 and blaIMP-1 in a multidrug-resistant Pseudomonas kurunegalensis (P. kurunegalensis) isolate obtained from human urine using whole-genome sequencing.
METHODS: This P. kurunegalensis isolate JC172 co-harbouring blaAFM-1 and blaIMP-1 underwent a comprehensive investigation that included antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing and bioinformatic analyses. To depict the evolutionary dynamics of P. kurunegalensis strains on a global scale, a phylogenetic tree was generated based on single nucleotide polymorphisms of core genomes.
RESULTS: JC172 was classified as P. kurunegalensis based on sequencing results and average nucleotide identity (ANI) assessments. This isolate was designated as ST114 and possesses a chromosome with 5 855 340 base pairs, exhibiting a GC content of 61.9%. Several resistance genes, including blaAFM-1 and blaIMP-1, blaOXA-246 and tmexC3D2-toprJ1 were identified on the chromosome. Genomic analysis revealed that blaAFM-1 was situated within a core module of the TnAs3-blaAFM-1 unit, which is bordered by class 1 integrons. This arrangement implies a significant potential for blaAFM-1 dissemination.
CONCLUSIONS: Our study revealed that blaAFM-1 and blaIMP-1, blaOXA-246 and tmexC3D2-toprJ1 were harboured by P. kurunegalensis. We suggested that ST114 P. kurunegalensis may serve as a reservoir for resistance genes.
METHODS: This P. kurunegalensis isolate JC172 co-harbouring blaAFM-1 and blaIMP-1 underwent a comprehensive investigation that included antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing and bioinformatic analyses. To depict the evolutionary dynamics of P. kurunegalensis strains on a global scale, a phylogenetic tree was generated based on single nucleotide polymorphisms of core genomes.
RESULTS: JC172 was classified as P. kurunegalensis based on sequencing results and average nucleotide identity (ANI) assessments. This isolate was designated as ST114 and possesses a chromosome with 5 855 340 base pairs, exhibiting a GC content of 61.9%. Several resistance genes, including blaAFM-1 and blaIMP-1, blaOXA-246 and tmexC3D2-toprJ1 were identified on the chromosome. Genomic analysis revealed that blaAFM-1 was situated within a core module of the TnAs3-blaAFM-1 unit, which is bordered by class 1 integrons. This arrangement implies a significant potential for blaAFM-1 dissemination.
CONCLUSIONS: Our study revealed that blaAFM-1 and blaIMP-1, blaOXA-246 and tmexC3D2-toprJ1 were harboured by P. kurunegalensis. We suggested that ST114 P. kurunegalensis may serve as a reservoir for resistance genes.