Journal of global antimicrobial resistance · 2025
Genomic and phylogenetic analysis of a carbapenem-resistant Raoultella ornithinolytica clinical isolate carrying blaOXA-181 from China.
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Zhu Zhiqiang, Zhao Xiaofei, Fan Zhaokun, Fu Ying, Li Xi, Ye Meizhen
Аннотация
OBJECTIVE: Raoultella ornithinolytica, a species within Enterobacteriaceae, is rarely multidrug-resistant. Here, we report a carbapenem-resistant R. ornithinolytica carrying blaOXA-181 and elucidate its genomic characteristics for the first time in China.
METHODS: Antimicrobial susceptibility testing and hybrid whole-genome sequencing were conducted on the R. ornithinolytica strain CRRAO31010. Comprehensive in silico analyses of virulence determinants, resistance genes, and their genetic contexts were performed. Conjugation experiments were conducted with Escherichia coli J53 as the recipient to assess the transferability of blaOXA-181. In addition, we combined our isolate with 56 R. ornithinolytica strains bearing the blaOXA-181 gene retrieved from the NCBI database for phylogenetic analysis.
RESULTS: R. ornithinolytica strain CRRAO31010 is a carbapenem-resistant strain characterized by its production of the OXA-181 enzyme, which confers resistance to multiple antibiotics. WGS revealed 22 antimicrobial resistance genes in CRRAO31010, including blaOXA-181 carried on a ColKP3-IncX3 hybrid plasmid. This plasmid was conjugated into E. coli J53 with a conjugation frequency of approximately 2.5 × 10-6 per donor cell. Phylogenetic analysis revealed that the earliest R. ornithinolytica strain carrying the blaOXA-181 gene was detected in the United Kingdom in 2019. The United States had the highest number of OXA-181-carrying R. ornithinolytica strains. Notably, IncX3-type plasmids had the highest prevalence (96.49%, 55/57) among these isolates.
CONCLUSIONS: We report the first carbapenem-resistant R. ornithinolytica strain harbouring the blaOXA-181 gene on a ColKP3-IncX3 hybrid plasmid from China. Given the high conjugative potential of IncX3 plasmids, vigilance is required to monitor the dissemination of such resistance determinants within R. ornithinolytica.
METHODS: Antimicrobial susceptibility testing and hybrid whole-genome sequencing were conducted on the R. ornithinolytica strain CRRAO31010. Comprehensive in silico analyses of virulence determinants, resistance genes, and their genetic contexts were performed. Conjugation experiments were conducted with Escherichia coli J53 as the recipient to assess the transferability of blaOXA-181. In addition, we combined our isolate with 56 R. ornithinolytica strains bearing the blaOXA-181 gene retrieved from the NCBI database for phylogenetic analysis.
RESULTS: R. ornithinolytica strain CRRAO31010 is a carbapenem-resistant strain characterized by its production of the OXA-181 enzyme, which confers resistance to multiple antibiotics. WGS revealed 22 antimicrobial resistance genes in CRRAO31010, including blaOXA-181 carried on a ColKP3-IncX3 hybrid plasmid. This plasmid was conjugated into E. coli J53 with a conjugation frequency of approximately 2.5 × 10-6 per donor cell. Phylogenetic analysis revealed that the earliest R. ornithinolytica strain carrying the blaOXA-181 gene was detected in the United Kingdom in 2019. The United States had the highest number of OXA-181-carrying R. ornithinolytica strains. Notably, IncX3-type plasmids had the highest prevalence (96.49%, 55/57) among these isolates.
CONCLUSIONS: We report the first carbapenem-resistant R. ornithinolytica strain harbouring the blaOXA-181 gene on a ColKP3-IncX3 hybrid plasmid from China. Given the high conjugative potential of IncX3 plasmids, vigilance is required to monitor the dissemination of such resistance determinants within R. ornithinolytica.