Anticancer research · 2021
Characteristics of Human Peripheral Blood γδ T Cells Expanded With Zoledronate.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Kim Minsong, Kim Hyori, Han Mina, Hwang Hyun Ju, Kim Hyery, Im Ho Joon, Kim Nayoung, Koh Kyung-Nam
Аннотация
BACKGROUND/AIM: This study aimed to investigate the characteristics of human peripheral blood γδ T cells, which were expanded ex vivo in the presence of zoledronate (ZOL).
MATERIALS AND METHODS: Human peripheral blood cells were cultured with IL-2 and IL-15 in the presence or absence of ZOL, which was added as a phospho-antigen, and their phenotypes were assessed by flow cytometry. Expanded γδ T cells were transduced with CD19 CAR vector, and the cytotoxicity was evaluated in vitro and in vivo by flow cytometry.
RESULTS: Ex vivo expansion did not hamper the expression of activating receptors. Interestingly, ZOL promoted the expression of CD226 (DNAM-1), TRAIL, and FAS-L in the Vδ1 subset of γδ T cells. Expanded γδ T cells containing CD19 CAR+ γδ T cells removed B cell lymphoma cells effectively in vivo.
CONCLUSION: γδ T cells could be a promising immunotherapeutic for cancer.
MATERIALS AND METHODS: Human peripheral blood cells were cultured with IL-2 and IL-15 in the presence or absence of ZOL, which was added as a phospho-antigen, and their phenotypes were assessed by flow cytometry. Expanded γδ T cells were transduced with CD19 CAR vector, and the cytotoxicity was evaluated in vitro and in vivo by flow cytometry.
RESULTS: Ex vivo expansion did not hamper the expression of activating receptors. Interestingly, ZOL promoted the expression of CD226 (DNAM-1), TRAIL, and FAS-L in the Vδ1 subset of γδ T cells. Expanded γδ T cells containing CD19 CAR+ γδ T cells removed B cell lymphoma cells effectively in vivo.
CONCLUSION: γδ T cells could be a promising immunotherapeutic for cancer.