[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 1997
[Promotive effects of basic fibroblast growth factor on proliferation of bovine lens epithelial cells].
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Li X, Yuan J, Kosaka
Аннотация
OBJECTIVE: In order to observe the effect of basic fibroblast growth factor (bFGF) on bovine lens epithelial cell growth.
METHODS: Primary culture and subculture of bovine lens epithelial cells were established in vitro first and then bFGF concentrated from 10(-2) ng/ml to 10(2) ng/ml was added to the epithelial cells of second passage respectively. The proliferative characteristics of the cells were measured with methyl thiazolyl tetrazolium (MTT) assay method.
RESULTS: It is indicated that in vitro bovine epithelial cells are characterized by easy adherence, rapid confluence, high growth, etc. After the treatment with bFGF, lens epithelial cells showed marked proliferation depending on the concentration of bFGF from 1 ng/ml to 10(2) ng/ml, especially in 10(2) ng/ml of bFGF.
CONCLUSION: It is suggested that bFGF play an important role in lens epithelial cell proliferation and posterior capsular opacities after cataract surgery.
METHODS: Primary culture and subculture of bovine lens epithelial cells were established in vitro first and then bFGF concentrated from 10(-2) ng/ml to 10(2) ng/ml was added to the epithelial cells of second passage respectively. The proliferative characteristics of the cells were measured with methyl thiazolyl tetrazolium (MTT) assay method.
RESULTS: It is indicated that in vitro bovine epithelial cells are characterized by easy adherence, rapid confluence, high growth, etc. After the treatment with bFGF, lens epithelial cells showed marked proliferation depending on the concentration of bFGF from 1 ng/ml to 10(2) ng/ml, especially in 10(2) ng/ml of bFGF.
CONCLUSION: It is suggested that bFGF play an important role in lens epithelial cell proliferation and posterior capsular opacities after cataract surgery.