Prevalence of Blastocystis sp. in freshwater fishes, poultry, and caprine from Penang, Malaysia.

Blastocystis is an enigmatic intestinal protist of humans and many animals. There is growing interest in its potential as an enteric pathogen, and the possible role of domestic and in-contact animals as reservoirs for human infection. The purpose of this study was to determine the prevalence of Blastocystis infection in fishes, poultry, and caprine in Penang, Malaysia. A total of 353 faecal samples/intestinal contents, consisting of 123 intestinal contents from freshwater fishes, 96 intestinal contents of commercially barn-reared chickens, 84 intestinal contents of barn-reared quails, and 50 faecal samples of caprine (29 from meat goats and 21 from dairy goats) were collected. Faecal sample/intestinal content from each animal was subjected to in-vitro cultivation method using Jones' medium supplemented with 10% horse serum. The respective colonization frequencies for dairy and meat goats were 47.6% and 31.0%, whereas 26.1% was recorded for chickens and 16.7% for quails. None of the freshwater fishes were found infected with Blastocystis. The organism was most commonly seen as spherically shaped vacuolated forms and cell diameter was significantly larger in poultry than in caprine. For further studies, molecular characterization of Blastocystis in poultry and livestock animals in the study area is highly recommended.