Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
Mobile Efficient Diagnostics of Infectious Diseases via On-Chip RT-qPCR: MEDIC-PCR.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Shrestha Kiran, Kim Seongryeong, Han Jiyeon, Florez Gabriela Morales, Truong Han, Hoang Trung, Parajuli Sajjan, Am Tiara, Kim Beomsoo, Jung Younsu, Abafogi Abdurhaman Teyib, Lee Yugyeong, Song Seung Hyun, Lee Jinkee, Park Sungsu, Kang Minhee, Huh Hee Jae, Cho Gyoujin, Lee Luke P
Аннотация
The COVID-19 outbreak has caused public and global health crises. However, the lack of on-site fast, reliable, sensitive, and low-cost reverse transcription polymerase chain reaction (RT-PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid mobile efficient diagnostics of infectious diseases via on-chip -RT-quantitative PCR (RT-qPCR): MEDIC-PCR. First, the authors use a roll-to-roll printing process to accomplish low-cost carbon-black-based disposable PCR chips that enable rapid LED-induced photothermal PCR cycles. The MEDIC-PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID-19 and 103 non-COVID-19 patients testing is completed by the MEDIC-PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC-PCR can contribute to the preventive global health in the face of a future pandemic.