Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy · 2025
Influence of Huaiqihuang granule on peritoneal fibrosis induced by peritoneal dialysis: A study based on rat model.
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Jia Lin, Liu Hui, Li Yongxia, Huang Yunfang
Аннотация
INTRODUCTION: To investigate the role of Huaiqihuang granule in the inhibition and immune regulation of peritoneal fibrosis in peritoneal dialysis rats.
METHODS: Peritoneal fibrosis rat models were established and subsequently assigned to five groups: a normal group (Group A); a model group (Group B); a low-concentration treatment group (Group C); a medium-concentration treatment group (Group D); and a high-concentration treatment group (Group E). The normal rats served as the control group (Group A). All rats underwent intragastric administration of Huaiqihuang granules for a duration of 21 days.
RESULTS: Peritoneal histopathological staining showed that group B rats had the highest degree of peritoneal fibrosis. In the intervention group, the degree of peritoneal fibrosis decreased with the increase of concentration of Huaiqihuang granule with a significant difference (p <0.05). Immunohistochemical analysis showed that the expression of TGF-β1, TNF-α, and α-SMA in rat peritoneal tissue was higher in group B than in group E (p< 0.05). Polymerase Chain Reaction (PCR) detection of mRNA expression of TGF-1 and fibronectin in rat peritoneal tissues: group B>group C>group D>group E>group A (p<0.05). The macrophage phagocytosis function, the levels of CD3+, CD4+, CD8+ T cells, and the CD4+/CD8+ ratio in peripheral blood were lowest in group B compared to groups C, D, and E (p < 0.05). In contrast, the levels of IL-2, IL-6, TGF-β1, and TNF-α were highest in group B (p < 0.05).
CONCLUSION: It is speculated that Huaiqihuang granule may be used clinically as effective drugs to improve peritoneal fibrosis and regulate immune function in peritoneal dialysis patients.
METHODS: Peritoneal fibrosis rat models were established and subsequently assigned to five groups: a normal group (Group A); a model group (Group B); a low-concentration treatment group (Group C); a medium-concentration treatment group (Group D); and a high-concentration treatment group (Group E). The normal rats served as the control group (Group A). All rats underwent intragastric administration of Huaiqihuang granules for a duration of 21 days.
RESULTS: Peritoneal histopathological staining showed that group B rats had the highest degree of peritoneal fibrosis. In the intervention group, the degree of peritoneal fibrosis decreased with the increase of concentration of Huaiqihuang granule with a significant difference (p <0.05). Immunohistochemical analysis showed that the expression of TGF-β1, TNF-α, and α-SMA in rat peritoneal tissue was higher in group B than in group E (p< 0.05). Polymerase Chain Reaction (PCR) detection of mRNA expression of TGF-1 and fibronectin in rat peritoneal tissues: group B>group C>group D>group E>group A (p<0.05). The macrophage phagocytosis function, the levels of CD3+, CD4+, CD8+ T cells, and the CD4+/CD8+ ratio in peripheral blood were lowest in group B compared to groups C, D, and E (p < 0.05). In contrast, the levels of IL-2, IL-6, TGF-β1, and TNF-α were highest in group B (p < 0.05).
CONCLUSION: It is speculated that Huaiqihuang granule may be used clinically as effective drugs to improve peritoneal fibrosis and regulate immune function in peritoneal dialysis patients.