Epigenomics · 2018
Alzheimer's disease DNA methylome of pyramidal layers in frontal cortex: laser-assisted microdissection study.
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Hernández Hernán Guillermo, Sandoval-Hernández Adrián Gabriel, Garrido-Gil Pablo, Labandeira-Garcia José Luis, Zelaya María Victoria, Bayon Gustavo F, Fernández Agustín F, Fraga Mario F, Arboleda Gonzalo, Arboleda Humberto
Аннотация
OBJECTIVE: To study DNA methylation patterns of cortical pyramidal layers susceptible to late-onset Alzheimer's disease (LOAD) neurodegeneration.
METHODS: Laser-assisted microdissection to select pyramidal layers' cells in frontal cortex of 32 human brains (18 LOAD) and Infinium DNA Methylation 450K analysis were performed to find differential methylated positions and regions, in addition to the corresponding gene set functional enrichment analyses.
RESULTS: Differential hypermethylation in several genomic regions and genes mainly in HOXA3, GSTP1, CXXC1-3 and BIN1. The functional enrichment analysis revealed genes significantly related to oxidative-stress and synapsis.
CONCLUSION: The present results indicate the differentially methylated genes related to neural projections, synapsis, oxidative stress and epigenetic regulator genes and represent the first epigenome of cortical pyramidal layers in LOAD.
METHODS: Laser-assisted microdissection to select pyramidal layers' cells in frontal cortex of 32 human brains (18 LOAD) and Infinium DNA Methylation 450K analysis were performed to find differential methylated positions and regions, in addition to the corresponding gene set functional enrichment analyses.
RESULTS: Differential hypermethylation in several genomic regions and genes mainly in HOXA3, GSTP1, CXXC1-3 and BIN1. The functional enrichment analysis revealed genes significantly related to oxidative-stress and synapsis.
CONCLUSION: The present results indicate the differentially methylated genes related to neural projections, synapsis, oxidative stress and epigenetic regulator genes and represent the first epigenome of cortical pyramidal layers in LOAD.
Предметные рубрики MeSH
Adaptor Proteins, Signal TransducingAgedAged, 80 and overAlzheimer DiseaseDNA MethylationDNA-Binding ProteinsFemaleFrontal LobeGlutathione S-Transferase piHomeodomain ProteinsHumansLaser Capture MicrodissectionMaleNuclear ProteinsOxidative StressPyramidal CellsSynaptic TransmissionTrans-ActivatorsTumor Suppressor Proteins