Медицинская библиотека · Epigenomics · 2018

Alzheimer's disease DNA methylome of pyramidal layers in frontal cortex: laser-assisted microdissection study.

Hernández Hernán Guillermo, Sandoval-Hernández Adrián Gabriel, Garrido-Gil Pablo, Labandeira-Garcia José Luis, Zelaya María Victoria, Bayon Gustavo F, Fernández Agustín F, Fraga Mario F, Arboleda Gonzalo, Arboleda Humberto

СтатьяПоддержка исследования: вне правительства СШАПеревод пока не подготовлен

Аннотация

OBJECTIVE

To study DNA methylation patterns of cortical pyramidal layers susceptible to late-onset Alzheimer's disease (LOAD) neurodegeneration.

METHODS

Laser-assisted microdissection to select pyramidal layers' cells in frontal cortex of 32 human brains (18 LOAD) and Infinium DNA Methylation 450K analysis were performed to find differential methylated positions and regions, in addition to the corresponding gene set functional enrichment analyses.

RESULTS

Differential hypermethylation in several genomic regions and genes mainly in HOXA3, GSTP1, CXXC1-3 and BIN1. The functional enrichment analysis revealed genes significantly related to oxidative-stress and synapsis.

CONCLUSION

The present results indicate the differentially methylated genes related to neural projections, synapsis, oxidative stress and epigenetic regulator genes and represent the first epigenome of cortical pyramidal layers in LOAD.

Для цитирования: Hernández Hernán Guillermo, Sandoval-Hernández Adrián Gabriel, Garrido-Gil Pablo, Labandeira-Garcia José Luis, Zelaya María Victoria, Bayon Gustavo F, Fernández Agustín F, Fraga Mario F, Arboleda Gonzalo, Arboleda Humberto. Alzheimer's disease DNA methylome of pyramidal layers in frontal cortex: laser-assisted microdissection study.. Epigenomics. 2018. DOI: 10.2217/epi-2017-0160.

Предметные рубрики MeSH

Adaptor Proteins, Signal Transducing Aged Aged, 80 and over Alzheimer Disease DNA Methylation DNA-Binding Proteins Female Frontal Lobe Glutathione S-Transferase pi Homeodomain Proteins Humans Laser Capture Microdissection Male Nuclear Proteins Oxidative Stress Pyramidal Cells Synaptic Transmission Trans-Activators Tumor Suppressor Proteins

Подготовка и перевод публикации

Обработка статьи · этапы, ошибки и управление

Загрузка состояния…

История · было → стало

Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.