Future microbiology · 2016
The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans.
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Oliveira Debora L, Fonseca Fernanda L, Zamith-Miranda Daniel, Nimrichter Leonardo, Rodrigues Jéssica, Pereira Marcos D, Reuwsaat Julia Cv, Schrank Augusto, Staats Charley, Kmetzsch Livia, Vainstein Marilene H, Rodrigues Marcio L
Аннотация
AIM: We investigated the involvement of the autophagy protein 7 (Atg7) in physiology and pathogenic potential of Cryptococcus neoformans.
MATERIALS & METHODS: The C. neoformans gene encoding Atg7 was deleted by biolistic transformation for characterization of autophagy mechanisms, pigment formation, cell dimensions, interaction with phagocytes and pathogenic potential in vivo.
RESULTS & CONCLUSION: ATG7 deletion resulted in defective autophagy mechanisms, enhanced pigmentation and increased cellular size both in vitro and in vivo. The atg7Δ mutant had decreased survival in the lung of infected mice, higher susceptibility to the killing machinery of different host phagocytes and reduced ability to kill an invertebrate host. These results connect Atg7 with mechanisms of pathogenicity in the C. neoformans model.
MATERIALS & METHODS: The C. neoformans gene encoding Atg7 was deleted by biolistic transformation for characterization of autophagy mechanisms, pigment formation, cell dimensions, interaction with phagocytes and pathogenic potential in vivo.
RESULTS & CONCLUSION: ATG7 deletion resulted in defective autophagy mechanisms, enhanced pigmentation and increased cellular size both in vitro and in vivo. The atg7Δ mutant had decreased survival in the lung of infected mice, higher susceptibility to the killing machinery of different host phagocytes and reduced ability to kill an invertebrate host. These results connect Atg7 with mechanisms of pathogenicity in the C. neoformans model.
Предметные рубрики MeSH
AnimalsArthropodsAutophagyAutophagy-Related Protein 7CryptococcosisCryptococcus neoformansDNA, FungalDisease Models, AnimalFemaleFungal ProteinsGene DeletionGene Expression Regulation, FungalGenes, FungalLarvaLungMiceMice, Inbred C57BLNitrogenOxygenPhagocytesPigments, BiologicalSequence DeletionSurvivalVirulence