Direct immunofluorescent studies of skin biopsies in pemphigus.

Direct immunofluorescent studies of skin biopsies from 16 pemphigus vulgaris patients revealed immunoglobulin deposits in 12 (75%) cases with fluorescence at intercellular areas in epidermis. In 2 patients (12.5%) dermoepidermal junction also showed immunoglobulin deposits. IgG was the commonest type of immunoglobulin demonstrated in 12 out of 16 (75%) cases followed by IgM in 5 (31.25%) and IgA in 1 (6.25%) cases. Seven (43.75%) cases showed presence of IgG alone while IgM with IgG was found in 4 (25%) cases. One (6.25%) case showed deposition of IgG, IgM and IgA. The results indicated that demonstration of immunoglobulin in skin biopsies by direct immunofluorescent technique is quite a useful adjunct in diagnostic confirmation of pemphigus.