The Laryngoscope · 2009
Roles of vascular endothelial growth factor, Angiopoietin 1, and Angiopoietin 2 in nasal polyp.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Park Seong Kook, Kim Hyeong In, Yang Young Il
Аннотация
OBJECTIVES/HYPOTHESIS: To determine the roles of vascular endothelial growth factor (VEGF), Angiopoietin (Ang)-1, and Ang-2 in nasal polyps (NPs) by assaying expression patterns and evaluating the effects of dexamethasone (DEX) on these factors in organ cultured NPs.
STUDY DESIGN: Prospective.
METHODS: Expression patterns of VEGF, Ang-1, and Ang-2 in NPs were compared with those in inferior turbinate mucosa samples. Tissue samples were analyzed using the enzyme-linked immunosorbent assay (ELISA) and immunofluorescent staining methods. To determine the effects of DEX, NP tissues were cultured using an air-liquid interface method. Cultures were maintained in the absence or presence of DEX (10 microM or 100 microM) for 24 hours, and tissue samples analyzed with ELISA.
RESULTS: VEGF and Ang-1 levels were significantly higher, whereas the Ang-2 level was significantly lower in NPs, compared to inferior turbinate mucosa (P < .05). In NPs, VEGF and Ang-1 were detected in glandular epithelial, vascular endothelial, as well as stromal inflammatory cells, whereas Ang-2 was detected only in stromal inflammatory cells. VEGF and Ang-1 levels were significantly lower, while Ang-2 levels were significantly higher in 100 microM DEX-treated group than nontreated group (P < .01).
CONCLUSIONS: Imbalance among VEGF, Ang 1, and Ang 2 may be important in the angiogenesis of NPs. Moreover, DEX can control the expression of these factors in NPs. We suggest that VEGF and Ang-1 act as positive regulatory factors, and Ang-2 functions as a negative regulatory factor of angiogenesis in NPs.
STUDY DESIGN: Prospective.
METHODS: Expression patterns of VEGF, Ang-1, and Ang-2 in NPs were compared with those in inferior turbinate mucosa samples. Tissue samples were analyzed using the enzyme-linked immunosorbent assay (ELISA) and immunofluorescent staining methods. To determine the effects of DEX, NP tissues were cultured using an air-liquid interface method. Cultures were maintained in the absence or presence of DEX (10 microM or 100 microM) for 24 hours, and tissue samples analyzed with ELISA.
RESULTS: VEGF and Ang-1 levels were significantly higher, whereas the Ang-2 level was significantly lower in NPs, compared to inferior turbinate mucosa (P < .05). In NPs, VEGF and Ang-1 were detected in glandular epithelial, vascular endothelial, as well as stromal inflammatory cells, whereas Ang-2 was detected only in stromal inflammatory cells. VEGF and Ang-1 levels were significantly lower, while Ang-2 levels were significantly higher in 100 microM DEX-treated group than nontreated group (P < .01).
CONCLUSIONS: Imbalance among VEGF, Ang 1, and Ang 2 may be important in the angiogenesis of NPs. Moreover, DEX can control the expression of these factors in NPs. We suggest that VEGF and Ang-1 act as positive regulatory factors, and Ang-2 functions as a negative regulatory factor of angiogenesis in NPs.