Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery · 2008
[Gene expression profiles in human nasal polyps studied by DNA microarray].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Liu Bing, Wu Jian, Fan Jingping, Peng Yucheng
Аннотация
OBJECTIVE: To explore the changes in gene expressions in the human nasal polyp.
METHOD: A 14500 gerie DNA microarray (Affymetrix) was used to examine gene expressions in 6 NP samples, 6 normal mucosal samples. The differentially expressed genes were identified and subjected to realtime PCR analysis.
RESULTS: The differentially expressed genes mostly involved in cytokines, adhesion genes about complements and their receptors, immune transcription regulatory molecules, signal transduction, differentially expressed genes including IL-8, RGS1, GRK4, CCL20, uteroglobin. The results of IL-8, RGS1 Real-time PCR of were consistent with that of gene chip analysis.
CONCLUSION: Microarray expression profile of NP samples is differential. RGS1 may play an important role in cell signal transduction, IL-8 may involve in occurrence of nasal polyps by inducing cells releasing inflammatory factors.
METHOD: A 14500 gerie DNA microarray (Affymetrix) was used to examine gene expressions in 6 NP samples, 6 normal mucosal samples. The differentially expressed genes were identified and subjected to realtime PCR analysis.
RESULTS: The differentially expressed genes mostly involved in cytokines, adhesion genes about complements and their receptors, immune transcription regulatory molecules, signal transduction, differentially expressed genes including IL-8, RGS1, GRK4, CCL20, uteroglobin. The results of IL-8, RGS1 Real-time PCR of were consistent with that of gene chip analysis.
CONCLUSION: Microarray expression profile of NP samples is differential. RGS1 may play an important role in cell signal transduction, IL-8 may involve in occurrence of nasal polyps by inducing cells releasing inflammatory factors.