Antifungal resistance of the Trichophyton mentagrophytes/Trichophyton interdigitale species complex: Insights from the China Antifungal Resistance Dermatophytes Surveillance network Study (CARDS).
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Kong Xue, Xie Wenting, Fu Meng, Feng Peiying, Li Zhihua, Liu Hongfang, Tong Zhongsheng, Abliz Palidae, Jiang Yanping, Yang Lianjuan, Yang Jie, Jing Dongyun, Sang Hong, Duan Xinsuo, Wang Miaomiao, Jiang Guan, Cao Cunwei, Cui Yan, Cai Qing, Dong Zhengbang, Dong Bilin, Dong Jiacheng, Gao Ying, Guo Yanyang, Ge Naicen, Hu Suquan, Liu Wei, Li Fuqiu, Liu Zehu, Li Meirong, Lu Sha, Liu Jia, Luo Wanglan, Mei Huan, Shi Dongmei, Tang Chao, Wang Chong, Wang Ying, Wang Xiaopeng, Wang Xiaodong, Xie Zhenmou, Zhang Siping, Zhu Min, Zhang Yu, Zhang Xiaoli, Zhang Jing, Zhan Ping, Zheng Hailin, She Xiaodong, Li Xiaofang, Liang Guanzhao, Liu Weida
Аннотация
OBJECTIVES: This study aims to evaluate the epidemiological features of antifungal-resistant TMTISC in China and monitor the prevalence of T. indotineae.
METHODS: A total of 230 TMTISC strains were isolated from nine tertiary hospitals across three economic regions of China. These isolates were identified through molecular sequencing and phylogenetic analysis. Additionally, in vitro antifungal susceptibility testing (AFST) was performed for eight drugs, following the EUCAST 11.0 protocol. Mutations in the squalene epoxidase (SQLE) gene were detected.
RESULTS: The phylogenetic tree revealed that 120 strains (52.17%) were T. mentagrophytes, 92 (40.0%) were T. interdigitale, and 18 (7.83%) were T. indotineae. Eight different genotypes were identified, including TM-IX, which was predominant in China, along with two newly discovered genotypes, TM-XXX and TM-XXXI. Among T. mentagrophytes isolates, tinea faciei (44.17%) was the common clinical type, primarily isolated from the central region. T. interdigitale isolates were mainly found in onychomycosis (36.96%), T. indotineae was commonly isolated from tinea corporis (94.44%) and tinea cruris (55.56%). AFST showed that all isolates of T. interdigitale and T. mentagrophytes were susceptible to terbinafine [minimum inhibitory concentrations (MICs) < 0.125 mg/L] and voriconazole (MICs < 0.5 mg/L). All isolates of T. indotineae were resistant to terbinafine (0.25 to >4.0 mg/L), exhibiting a single amino acid substitution in SQLE (Phe397Leu, Leu393Ser or Phe415Cys). Additionally, elevated MICs were observed in TMTISC for itraconazole, miconazole, ciclopirox (1-2 mg/L) and griseofulvin (1-64 mg/L).
CONCLUSIONS: The TMTISC was predominantly composed of T. mentagrophytes, followed by T. interdigitale and T. indotineae. A rising trend of reduced azoles susceptibility in TMTISC and multi-drug resistance among T. indotineae isolates has been observed in China.