A novel gdmH-related gene, ghl, involved in environmental stress tolerance and vancomycin susceptibility in milk-derived Staphylococcus aureus.

Staphylococcus aureus is one of the main microorganisms that contaminate dairy products and pickled foods, and has a great impact on economy and human health. GdmH-related proteins, as important functional units widely present in Staphylococcus species, have not been reported in S. aureus so far. In this study, we identified a gdmH-related gene, named ghl. We found that mutation of ghl gene could decrease the tolerance of environmental stresses (heat, desiccation, salt and hydrogen peroxide) of S. aureus and enhanced the capacities of biofilm formation. In addition, the ghl mutant was more sensitive to vancomycin on CAMHB solid plates but more resistant to vancomycin in CAMHB liquid medium compared to wild type RMSA24. These results indicated that ghl is an important factor to respond to environmental stress in foodborne S. aureus. This paper for the first time reported that a GdmH-related protein plays an important role in environmental tolerance, providing a new direction for the follow-up study of GdmH-related proteins, as well as a potential target gene for further research on the tolerance mechanism of Staphylococcus aureus in food processing and the control of biofilm formation.