Pakistan journal of biological sciences : PJBS · 2017
Studies on Staphylococcus aureus Isolated from Pimples.
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Adetutu Ajayi Adesola, Oritsewehinmi Bibi, Ikhiwili Oniha Margaret, Moradeke Atolagbe Oluwabunmi, Odochi Anosike Selina, Adeola Onibokun Elizabeth
Аннотация
BACKGROUND AND OBJECTIVE: Pimples (acne) are small skin lesions or inflammations of the skin. The most common factor causing acne is the hormonal changes that occur during adolescent and teenage years. Antibiotics are becoming less effective in the treatment of pimples due to increasing concerns of antibiotic resistance. This study was therefore carried out to characterize the isolates from the pimples of Covenant University Students and to determine their antibiotics sensitivity pattern.
MATERIALS AND METHODS: A total of 20 swab samples were obtained from male and female students with obvious signs of pimples in Covenant University, Ota, Ogun State, Nigeria. The samples obtained were cultured on Mannitol Salt Agar and incubated at 37°C. Pure isolates obtained were subjected to Gram staining and other biochemical tests for identification. The isolates were further subjected to antibiotics sensitivity tests using antibiotic dics.
RESULTS: Macroscopic examination indicated that the organisms were convex, smooth and shiny. Microscopic examination revealed that the isolates were positive after employing the Gram Staining technique and they appeared as grape-like clusters. Biochemical tests revealed that the isolates were Coagulase positive, Catalase positive, Urease positive, Citrate positive, Methyl-Red positive, Voges-Proskauer negative and negative upon starch hydrolysis. The sugar fermentation tests revealed that the isolates fermented Glucose, Maltose, Galactose, Sucrose and Lactose, respectively. The antibiotic susceptibility test showed that isolates were resistant to Cotrimazole, Cloxacillin, Erythromycin, Gentamycin, Augmentin, Streptomycin, Tetracycline and Chloramphenicol.
CONCLUSION: The results therefore indicated that the isolates were Staphylococcus aureus and other staphylococci species. Indiscriminate use of antibiotics should be avoided to prevent the development of resistant strains of the Staphylococci genera and other pathogenic organisms.
MATERIALS AND METHODS: A total of 20 swab samples were obtained from male and female students with obvious signs of pimples in Covenant University, Ota, Ogun State, Nigeria. The samples obtained were cultured on Mannitol Salt Agar and incubated at 37°C. Pure isolates obtained were subjected to Gram staining and other biochemical tests for identification. The isolates were further subjected to antibiotics sensitivity tests using antibiotic dics.
RESULTS: Macroscopic examination indicated that the organisms were convex, smooth and shiny. Microscopic examination revealed that the isolates were positive after employing the Gram Staining technique and they appeared as grape-like clusters. Biochemical tests revealed that the isolates were Coagulase positive, Catalase positive, Urease positive, Citrate positive, Methyl-Red positive, Voges-Proskauer negative and negative upon starch hydrolysis. The sugar fermentation tests revealed that the isolates fermented Glucose, Maltose, Galactose, Sucrose and Lactose, respectively. The antibiotic susceptibility test showed that isolates were resistant to Cotrimazole, Cloxacillin, Erythromycin, Gentamycin, Augmentin, Streptomycin, Tetracycline and Chloramphenicol.
CONCLUSION: The results therefore indicated that the isolates were Staphylococcus aureus and other staphylococci species. Indiscriminate use of antibiotics should be avoided to prevent the development of resistant strains of the Staphylococci genera and other pathogenic organisms.