Pakistan journal of pharmaceutical sciences · 2026
Exploring pulegone in type 2 diabetes treatment: Molecular docking, influence on insulin resistance and modulation of pro/anti-inflammatory cytokines.
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Muhammad Ali Rubina, Abdo Albajali Abdullah, Mobashar Aisha, Maqbool Tahir, Shabbir Arham, Barkat Kashif, Hussain Khalid
Аннотация
BACKGROUND: Type 2 diabetes involves insulin resistance, where muscle, liver, and fat cells fail to utilize glucose leading to hyperglycemia.
OBJECTIVES: This study explores the therapeutic potential of pulegone in type 2 diabetes by examining its molecular interactions with key metabolic targets and evaluating its effects on insulin resistance and inflammatory cytokine modulation.
METHODS: Except for normal controls, animals were fed a high-sucrose, high-fat diet for four months to induce insulin resistance. Treatment groups received metformin (150 mg/kg) or pulegone doses: low (5 mg/kg), medium (10 mg/kg), and high (15 mg/kg) for 45 days. Post-treatment, tissues and blood were collected. Blood serum was analyzed for liver markers, lipid profile, and blood for glycated hemoglobin. ELISA was used for cytokines (resistin, adiponectin, IL-1Ra, TNF-α), and qPCR assessed gene expression of NF-κB, TNF-α, PPAR-α/γ, IL-6, IL-10, IL-1Ra, and adiponectin. Histopathology was performed. Data were analyzed using GraphPad Prism. Molecular docking was conducted to identify the binding affinities of pulegone to target proteins.
RESULTS: Pulegone significantly reduced fasting blood glucose, glycated hemoglobin, triglycerides, cholesterol, and liver enzymes, while increasing HDL levels compared with diabetic controls. It also decreased serum resistin, TNF-α, IL-6 and NF-κB expression, while upregulating adiponectin, IL-10, PPAR-α, PPAR-γ and IL-1Ra. Histopathological examination showed protective effects on the pancreas, liver, kidney, heart, and aorta. Docking analysis favored strong binding affinities of pulegone with key targets.
CONCLUSION: Pulegone ameliorates insulin resistance by improving glycemic and lipid profiles, modulating pro- and anti-inflammatory cytokines, and protecting vital organs, suggesting its potential as a multitarget antidiabetic agent.
OBJECTIVES: This study explores the therapeutic potential of pulegone in type 2 diabetes by examining its molecular interactions with key metabolic targets and evaluating its effects on insulin resistance and inflammatory cytokine modulation.
METHODS: Except for normal controls, animals were fed a high-sucrose, high-fat diet for four months to induce insulin resistance. Treatment groups received metformin (150 mg/kg) or pulegone doses: low (5 mg/kg), medium (10 mg/kg), and high (15 mg/kg) for 45 days. Post-treatment, tissues and blood were collected. Blood serum was analyzed for liver markers, lipid profile, and blood for glycated hemoglobin. ELISA was used for cytokines (resistin, adiponectin, IL-1Ra, TNF-α), and qPCR assessed gene expression of NF-κB, TNF-α, PPAR-α/γ, IL-6, IL-10, IL-1Ra, and adiponectin. Histopathology was performed. Data were analyzed using GraphPad Prism. Molecular docking was conducted to identify the binding affinities of pulegone to target proteins.
RESULTS: Pulegone significantly reduced fasting blood glucose, glycated hemoglobin, triglycerides, cholesterol, and liver enzymes, while increasing HDL levels compared with diabetic controls. It also decreased serum resistin, TNF-α, IL-6 and NF-κB expression, while upregulating adiponectin, IL-10, PPAR-α, PPAR-γ and IL-1Ra. Histopathological examination showed protective effects on the pancreas, liver, kidney, heart, and aorta. Docking analysis favored strong binding affinities of pulegone with key targets.
CONCLUSION: Pulegone ameliorates insulin resistance by improving glycemic and lipid profiles, modulating pro- and anti-inflammatory cytokines, and protecting vital organs, suggesting its potential as a multitarget antidiabetic agent.