PloS one · 2026
Feasibility of tumor-informed circulating tumor DNA for detecting minimal residual disease in surgically resected biliary tract cancer.
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Park Younghee, Kim Kyung Su, Jo Hyunji, Kim Hwang-Phil, Kyung Dong Soo, Yoo Youngeun, Min Seog Ki, Nam Eun Mi, Kim Kyubo
Аннотация
BACKGROUND: We prospectively investigated the potential of tumor-informed circulating tumor DNA (ctDNA) for detecting minimal residual disease (MRD) in surgically resected biliary tract cancer (BTC).
METHODS: Personalized panels were developed using individual variants identified from the whole-exome sequencing of surgical specimens from each patient. Two sequential blood samples, collected preoperatively and within 6 weeks after surgery, were analyzed. A positive ctDNA result was defined as the identification of two or more patient-specific mutations.
RESULTS: A total of 18 patients were enrolled. However, 1 patient was excluded due to inoperability detected during surgery, and personalized target panels could not be created for 3 patients due to a low number of target variants, resulting in the analysis of 14 patients. There was a tendency for a higher preoperative ctDNA positivity rate in an advanced overall stage (100% in stage III-IV vs. 44.4% in stage I-II, p = 0.126) and node-positive disease (100% in node-positive vs. 60% in node-negative, p = 0.210). After a median follow-up of 17.4 months (range, 11.4-31.1), the 1-year and 2-year progression-free survival (PFS) rates were 78.6% and 58.2%, respectively. Changes in ctDNA positivity status were negative to negative in 3 patients (25.0%), positive to negative in 3 patients (25.0%), and positive to positive in 6 patients (50%). Additionally, there was a trend for an association between poorer PFS and both preoperative and postoperative ctDNA positivity. Among ctDNA-negative patients before surgery, no progression was observed; however, 5 out of 10 ctDNA-positive patients experienced progression. Postoperatively, only 1 out of 6 ctDNA-negative patients experienced progression, in contrast to 3 out of 6 patients with a positive ctDNA result.
CONCLUSIONS: Although limited by the small sample size, our results may indicate a possible role for tumor-informed ctDNA analysis in detecting MRD in surgically resected BTC and warrant further validation in larger studies.
METHODS: Personalized panels were developed using individual variants identified from the whole-exome sequencing of surgical specimens from each patient. Two sequential blood samples, collected preoperatively and within 6 weeks after surgery, were analyzed. A positive ctDNA result was defined as the identification of two or more patient-specific mutations.
RESULTS: A total of 18 patients were enrolled. However, 1 patient was excluded due to inoperability detected during surgery, and personalized target panels could not be created for 3 patients due to a low number of target variants, resulting in the analysis of 14 patients. There was a tendency for a higher preoperative ctDNA positivity rate in an advanced overall stage (100% in stage III-IV vs. 44.4% in stage I-II, p = 0.126) and node-positive disease (100% in node-positive vs. 60% in node-negative, p = 0.210). After a median follow-up of 17.4 months (range, 11.4-31.1), the 1-year and 2-year progression-free survival (PFS) rates were 78.6% and 58.2%, respectively. Changes in ctDNA positivity status were negative to negative in 3 patients (25.0%), positive to negative in 3 patients (25.0%), and positive to positive in 6 patients (50%). Additionally, there was a trend for an association between poorer PFS and both preoperative and postoperative ctDNA positivity. Among ctDNA-negative patients before surgery, no progression was observed; however, 5 out of 10 ctDNA-positive patients experienced progression. Postoperatively, only 1 out of 6 ctDNA-negative patients experienced progression, in contrast to 3 out of 6 patients with a positive ctDNA result.
CONCLUSIONS: Although limited by the small sample size, our results may indicate a possible role for tumor-informed ctDNA analysis in detecting MRD in surgically resected BTC and warrant further validation in larger studies.